H&E staining for Leica Autostainer
1		Oven	15 min
2	1	xylene	45 sec
3	2	xylene	45 sec
4	3	xylene	1 min
5	4	EtOH100%	30 sec
6	5	EtOH100%	30 sec
7	6	EtOH95%	30 sec
8	7	EtOH95%	30 sec
9	Wash 1	Water	1 min
10	8	Hematoxylin	5 min	Gill3
11	Wash 2	Water	1 min
12	9	Clarifier II	15 sec
13	Wash 3	Water	1 min 15 sec
14	10	Bluing	30 sec
15	Wash 4	Water	30 sec
16	11	EtOH80%	30 sec
17	12	Eosin	30 sec
18	13	EtOH95%	30 sec
19	14	EtOH100%	30 sec
20	15	EtOH100%	30 sec
21	16	EtOH100%	30 sec
22	17	EtOH100%	30 sec
23	18	xylene	30 sec
24	Exit	xylene